NUDT8 Polyclonal antibody

NUDT8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16098-1-AP

Synonyms

Nudix motif 8, NUDT8

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Validation Data Gallery

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  WB analysis using 16098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human colon cancer tissue, human heart tissue, human kidney tissue, human skin tissue, mouse heart tissue, human liver tissue, human spleen tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16098-1-AP targets NUDT8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT8 fusion protein Ag8945
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 8
• Calculated Molecular Weight: 236 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC018644
• Gene Symbol: NUDT8
• Gene ID (NCBI): 254552
• RRID: AB_2154132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.