NUDT21 Polyclonal antibody

• Featured Product
• KD/KO Validated

NUDT21 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 10322-1-AP

Synonyms

Nudix hydrolase 21, Nucleoside diphosphate-linked moiety X motif 21, CPSF5, CPSF25, Cleavage factor Im complex 25 kDa subunit

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 10322-1-AP

  WB result of NUDT21 antibody (10322-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDT21 transfected NIH/3T3 cells.

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  WB analysis using 10322-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10322-1-AP (NUDT21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10322-1-AP

  IP result of anti-NUDT21 (IP:10322-1-AP, 4ug; Detection:10322-1-AP 1:2000) with MCF-7 cells lysate 640 ug.

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  IHC staining of human colon cancer using 10322-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10322-1-AP (NUDT21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10322-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10322-1-AP (NUDT21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10322-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (10322-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HL-60 cells, NIH/3T3 cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 29 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Product Information

10322-1-AP targets NUDT21 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT21 fusion protein Ag0393
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 21
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC001403
• Gene Symbol: NUDT21
• Gene ID (NCBI): 11051
• RRID: AB_2251496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

Publications

Species	Application	Title
human	WB	Nature; CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.; Authors - Chioniso P Masamha ; KD Validated
human	WB	Mol Cell; A Complex of U1 snRNP with Cleavage and Polyadenylation Factors Controls Telescripting, Regulating mRNA Transcription in Human Cells.; Authors - Byung Ran So ; KD Validated
	WB	Mol Cell; Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors.; Authors - Ashish Misra
human	WB	Sci Adv; Alternative polyadenylation is a determinant of oncogenic Ras function.; Authors - Aishwarya Subramanian
human	IHC	J Exp Med; Downregulation of CFIm25 amplifies dermal fibrosis through alternative polyadenylation.; Authors - Tingting Weng
mouse	WB	EMBO J; The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination.; Authors - Xiao Yao

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