NUDT18 Polyclonal antibody

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  IP experiment of THP-1 using 17318-1-AP

  IP result of anti-NUDT18 (IP:17318-1-AP, 4ug; Detection:17318-1-AP 1:500) with THP-1 cells lysate 2480 ug.

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  IF Staining of THP-1 using 17318-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using NUDT18 antibody (17318-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

NUDT18 Polyclonal Antibody for IF/ICC, IP, ELISA

Cat No. 17318-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Synonyms

MTH3, Nucleoside diphosphate-linked moiety X motif 18, EC:3.6.1.58, 8-oxo-dGDP phosphatase NUDT18, 7,8-dihydro-8-oxoguanine phosphatase

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Tested Applications

• Positive IP detected in: THP-1 cells
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17318-1-AP targets NUDT18 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT18 fusion protein Ag11292
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 18
• Calculated Molecular Weight: 323 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC016902
• Gene Symbol: NUDT18
• Gene ID (NCBI): 79873
• RRID: AB_3669267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZVK8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

8-oxo-dGDP phosphatase NUDT18, also known as MTH3, belongs to the Nudix hydrolase family that eliminate potentially toxic nucleotide metabolites from the cell and regulate the concentrations and availability of various nucleotide substrates, cofactors, and signaling molecules (PMID: 35732208, 22556419). NUDT18 is specifically active against 8-oxo-dGDP and hardly cleaves 8-oxo-dGTP. NUDT18 is markedly elevated expression in noncancerous cell lines in comparison to cancerous counterpart, and NUDT18 deficient cells have been reported to exhibit a noticeably reduced proliferation rate (PMID: 38944687).