NUDT12 Polyclonal antibody

NUDT12 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (2)

Cat no : 17487-1-AP

Synonyms

Nucleoside diphosphate-linked moiety X motif 12, NADH pyrophosphatase NUDT12, NAD-capped RNA hydrolase NUDT12, EC:3.6.1.22, EC:3.6.1.-

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Validation Data Gallery

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  WB analysis using 17487-1-AP

  mouse liver tissues were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17487-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17487-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17487-1-AP (NUDT12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17487-1-AP targets NUDT12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT12 fusion protein Ag11557
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 12
• Calculated Molecular Weight: 462 aa, 52 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC041099
• Gene Symbol: NUDT12
• Gene ID (NCBI): 83594
• RRID: AB_2236164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Dis; A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells.; Authors - Hiroaki Konishi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (11-19-2024)

Antibody detect a band with the expected molecular weight of Nudt12

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

NUDT12 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:17487-1-AP)

FH

Moritz (Verified Customer) (09-12-2024)

worked well for detecting NUDT12 in WB. band was specific and slightly below 50kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

NUDT12 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:17487-1-AP)