NUCB2/nesfatin-1 Recombinant antibody

NUCB2/nesfatin-1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241037D8

Cat no : 83917-2-RR

Synonyms

NUCB2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA

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Validation Data Gallery

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  WB analysis using 83917-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83917-2-RR (NUCB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83917-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83917-2-RR (NUCB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 83917-2-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR, Clone:241037D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HepG2 using 83917-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR, Clone:241037D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83917-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83917-2-RR against Human NUCB2/nesfatin-1 were performed. The affinity constant is 8.99 nM.

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse testis tissue, C6 cells, mouse spleen tissue, mouse brain tissue, rat brain tissue
• Positive FC (Intra) detected in: HepG2 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83917-2-RR is NUCB2/nesfatin-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NUCB2/nesfatin-1 fusion protein Ag25152
• Full Name: nucleobindin 2
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_005013
• Gene Symbol: NUCB2
• Gene ID (NCBI): 4925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).