NUCB2/nesfatin-1 Polyclonal antibody, PBS Only

NUCB2/nesfatin-1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30604-1-PBS

Synonyms

NUCB2, Nucleobindin-2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4

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Validation Data Gallery

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  WB analysis using 30604-1-AP (same clone as 30604-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30604-1-AP (same clone as 30604-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 30604-1-AP (same clone as 30604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 30604-1-AP (same clone as 30604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 30604-1-AP (same clone as 30604-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NUCB2/nesfatin-1 antibody (30604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

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Product Information

30604-1-PBS targets NUCB2/nesfatin-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUCB2/nesfatin-1 fusion protein Ag30704
• Full Name: nucleobindin 2
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: NM_005013
• Gene Symbol: NUCB2
• Gene ID (NCBI): 4925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).