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NUCB2/nesfatin-1 Polyclonal antibody

NUCB2/nesfatin-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30604-1-AP

Synonyms

NUCB2, Nucleobindin-2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    WB analysis using 30604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    WB analysis using 30604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30604-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30604-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    IF Staining of U2OS using 30604-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NUCB2/nesfatin-1 antibody (30604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inmouse brain tissue, human milk, SGC-7901 cells, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30604-1-AP targets NUCB2/nesfatin-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUCB2/nesfatin-1 fusion protein Ag30704
Full Name nucleobindin 2
Calculated Molecular Weight 50 kDa
Observed Molecular Weight46-50 kDa
GenBank Accession NumberNM_005013
Gene Symbol NUCB2
Gene ID (NCBI) 4925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

Protocols

Product Specific Protocols
WB protocol for NUCB2/nesfatin-1 antibody 30604-1-APDownload protocol
IHC protocol for NUCB2/nesfatin-1 antibody 30604-1-APDownload protocol
IF protocol for NUCB2/nesfatin-1 antibody 30604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols