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NUBP1 Polyclonal antibody

NUBP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18011-1-AP

Synonyms

Nucleotide binding protein 1, Nbp35, NBP1, NBP 1, NBP



Tested Applications

Positive WB detected inA549 cells, HeLa cells, LNCaP cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18011-1-AP targets NUBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUBP1 fusion protein Ag12510
Full Name nucleotide binding protein 1 (MinD homolog, E. coli)
Calculated Molecular Weight 309 aa, 33 kDa
Observed Molecular Weight36-40 kDa
GenBank Accession NumberBC100290
Gene Symbol NUBP1
Gene ID (NCBI) 4682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NUBP1 antibody 18011-1-APDownload protocol
IHC protocol for NUBP1 antibody 18011-1-APDownload protocol
IP protocol for NUBP1 antibody 18011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer

Authors - Subhadip Mukhopadhyay