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NUAK2 Recombinant antibody

NUAK2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242220C1

Cat no : 84849-4-RR

Synonyms

OMPHK2, NUAK family SNF1-like kinase 2, NUAK 2, EC:2.7.11.1, ANPH2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUAK2 Recombinant antibody (84849-4-RR)

    WB analysis using 84849-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84849-4-RR (NUAK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84849-4-RR

    Affinity and Kinetic Characterization of 84849-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84849-4-RR against Human NUAK2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, SGC-7901 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84849-4-RR targets NUAK2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUAK2 fusion protein Ag35302
Full Name NUAK family, SNF1-like kinase, 2
Calculated Molecular Weight70kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_030952
Gene Symbol NUAK2
Gene ID (NCBI) 81788
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUAK2, also known as OMPHK2 and SNARK, is the fourth member of the AMPK kinase family. The molecular mass of NUAK2 is 69 kDa and it plays a key role in neural tube closure during embryonic development (PMID: 32845958).

Protocols

Product Specific Protocols
WB protocol for NUAK2 antibody 84849-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols