NTB-A/SLAMF6 Polyclonal antibody, PBS Only

NTB-A/SLAMF6 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22711-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of Daudi using 22711-1-AP (same clone as 22711-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 22711-1-AP (SLAMF6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22711-1-AP (same clone as 22711-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22711-1-AP (same clone as 22711-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22711-1-AP (same clone as 22711-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22711-1-AP (SLAMF6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22711-1-AP (same clone as 22711-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22711-1-AP (SLAMF6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

---

Product Information

22711-1-PBS targets NTB-A/SLAMF6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NTB-A/SLAMF6 fusion protein Ag18517
• Full Name: SLAM family member 6
• Calculated Molecular Weight: 332 aa, 37 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC114495
• Gene Symbol: SLAMF6
• Gene ID (NCBI): 114836
• RRID: AB_2879154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Signaling lymphocyte activation molecule 6 (SLAMF6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules (PMID:31199820). SLAMF6 is a type I transmembrane protein with two extracellular immunoglobins (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs, one of which is immunoreceptor tyrosine-based switch motif (PMID:31315913). SLAMF6 is expressed on a wide variety of immune cells including T cells (also TFH), B cells, NK cells (expressed in humans only), double positive thymocytes, eosinophils, and neutrophils (mouse only) (PMID:11862385).