PNAD Polyclonal antibody

• 

  WB analysis using 17132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17132-1-AP (PNAD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

PNAD Polyclonal Antibody for WB, ELISA

Cat No. 17132-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NTAN1, EC:3.5.1.121, N terminal asparagine amidase, PNAA, Protein NH2-terminal asparagine amidohydrolase

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, L02 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17132-1-AP targets PNAD in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PNAD fusion protein Ag10880
• Full Name: N-terminal asparagine amidase
• Calculated Molecular Weight: 310 aa, 35 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC017336
• Gene Symbol: NTAN1
• Gene ID (NCBI): 123803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96AB6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PNAD is an N-terminal aspartic acid deamidation enzyme, which can deamidate N-terminal aspartic acid residues to aspartic acid, making the protein prone to arginination, polyubiquitination and degradation. In addition, PNAD is involved in the regulation of apoptosis by mediating the degradation of the C-terminal fragment of Drosophila apoptosis suppressor protein 1.