NT5E/CD73 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NT5E/CD73 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5G8

Cat no : 67789-1-PBS

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E,CD73, NTE

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Validation Data Gallery

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  WB analysis of HepG2 using 67789-1-Ig (same clone as 67789-1-PBS)

  WB result of NT5E/CD73 antibody (67789-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5E,CD73 transfected HepG2 cells. This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  WB analysis using 67789-1-Ig (same clone as 67789-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67789-1-PBS targets NT5E/CD73 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NT5E/CD73 fusion protein Ag30616
• Full Name: 5'-nucleotidase, ecto (CD73)
• Calculated Molecular Weight: 29 kDa, 63 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC015940
• Gene Symbol: CD73
• Gene ID (NCBI): 4907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).