NT5C2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NT5C2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15223-1-AP

Synonyms

Cytosolic IMP/GMP-specific 5'-nucleotidase, Cytosolic 5'-nucleotidase II, Cytosolic 5 nucleotidase II, cN-II, cN II

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Validation Data Gallery

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  WB analysis using 15223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15223-1-AP

  WB result of NT5C2 antibody (15223-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5C2 transfected A549 cells.

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  WB analysis of human placenta using 15223-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 15223-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NT5C2 antibody (15223-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human placenta tissue, A549 cells, NCl-H1299 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, NCI-H1299 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

15223-1-AP targets NT5C2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NT5C2 fusion protein Ag7438
• Full Name: 5'-nucleotidase, cytosolic II
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC001595
• Gene Symbol: NT5C2
• Gene ID (NCBI): 22978
• RRID: AB_2153946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	BMC Med Genomics; Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.; Authors - Xing-Cheng Zhao
mouse	WB,IHC	Cell Death Dis; Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation; Authors - Mei-Xin Li