NT5C1A Polyclonal antibody

• 

  WB analysis using 21284-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21284-1-AP (NT5C1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

NT5C1A Polyclonal Antibody for WB, ELISA

Cat No. 21284-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

5' nucleotidase, cytosolic IA, CN I, CN IA, CN1, CN1A, Cytosolic 5 nucleotidase 1A, Cytosolic 5 nucleotidase IA, NT5C1A

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Tested Applications

• Positive WB detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21284-1-AP targets NT5C1A in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NT5C1A fusion protein Ag15802
• Full Name: 5'-nucleotidase, cytosolic IA
• Calculated Molecular Weight: 368 aa, 41 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC103880
• Gene Symbol: NT5C1A
• Gene ID (NCBI): 84618
• RRID: AB_3085649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXI3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NT5C1A (5′-nucleotidase cytosolic 1A) is a 365 amino acid protein belonging to the 5′-nucleotidase type 3 family. AMP can be deaminated by AMP-deaminase (AMPD) to IMP, which is hydrolyzed to inosine by cytosolic 5'-nucleotidase II (NT5C2). AMP can also be hydrolyzed to adenosine by cytosolic 5'-nucleotidase 1A (NT5C1A). NT5C1A also assists in the regulation of adenosine levels in heart during ischemia and hypoxia.