NSUN7 Polyclonal antibody

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  WB analysis using 29787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29787-1-AP (NSUN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NSUN7 Polyclonal Antibody for WB, ELISA

Cat No. 29787-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FLJ14001

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29787-1-AP targets NSUN7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSUN7 fusion protein Ag31860
• Full Name: NOL1/NOP2/Sun domain family, member 7
• Calculated Molecular Weight: 718 aa, 81 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC036568
• Gene Symbol: NSUN7
• Gene ID (NCBI): 79730
• RRID: AB_2923609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).