NSUN7 Polyclonal antibody

NSUN7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17546-1-AP

Synonyms

NSUN7

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Validation Data Gallery

• 

  WB analysis using 17546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat testis using 17546-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17546-1-AP (NSUN7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse testis tissue, rat liver tissue
• Positive IHC detected in: rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17546-1-AP targets NSUN7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSUN7 fusion protein Ag11650
• Full Name: NOL1/NOP2/Sun domain family, member 7
• Calculated Molecular Weight: 475 aa, 54 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC036568
• Gene Symbol: NSUN7
• Gene ID (NCBI): 79730
• RRID: AB_2878415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Oncol; Comprehensive Analysis of 5-Methylcytosine (m5C) Regulators and the Immune Microenvironment in Pancreatic Adenocarcinoma to Aid Immunotherapy.; Authors - Ronglin Wang