NSUN6 Monoclonal antibody

NSUN6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1H8

Cat no : 68614-1-Ig

Synonyms

4933414E04Rik, FLJ23743, NOPD1, NSUN6

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Validation Data Gallery

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  WB analysis using 68614-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68614-1-Ig (NSUN6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, Mo7e cells, Ramos cells, Raji cells, pig liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68614-1-Ig targets NSUN6 in WB, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NSUN6 fusion protein Ag11084
• Full Name: NOL1/NOP2/Sun domain family, member 6
• Calculated Molecular Weight: 469 aa, 52 kDa
• Observed Molecular Weight: 50-52 kDa
• GenBank Accession Number: BC035778
• Gene Symbol: NSUN6
• Gene ID (NCBI): 221078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with the Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.