NSUN5 Recombinant antibody, PBS Only (Detector)

NSUN5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242168D5

Cat no : 84768-2-PBS

Synonyms

NOL1-related protein, NOL1R, NOL1, EC:2.1.1.-, 28S rRNA (cytosine-C(5))-methyltransferase

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Validation Data Gallery

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  WB analysis using 84768-2-RR (same clone as 84768-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84768-2-RR (NSUN5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84768-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84768-2-RR (same clone as 84768-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84768-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84768-2-RR (same clone as 84768-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84768-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01548-1

  Cytometric bead array standard curve of MP01548-1, NSUN5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84768-1-PBS. Detection antibody: 84768-2-PBS. Standard: Ag7711. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84768-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84768-2-RR against Human NSUN5 were performed. The affinity constant is 0.69 nM.

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Product Information

84768-2-PBS targets NSUN5 as part of a matched antibody pair:

MP01548-1: 84768-1-PBS capture and 84768-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NSUN5 fusion protein Ag7711
• Full Name: NOL1/NOP2/Sun domain family, member 5
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47~51 kDa
• GenBank Accession Number: BC008084
• Gene Symbol: NSUN5
• Gene ID (NCBI): 55695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NSUN5 is a conserved RNA methyltransferase belonging to the Nop2/SUN domain family, which is highly conserved from archaea to eukaryotes and has close contact with RNA methylation because of their S-adenosyl methionine binding-domain. NSUN5 was a promoter in the progression of CRC mainly through cell cycle regulation (PMID: 32774740). Loss of NSUN5 induces growth phenotypes in mice and different mammalian cells, which is likely connected to the requirement of NSUN5 for ribosomal RNA modification and normal global protein translation, especially in highly proliferative cells (PMID: 31722427). Western blot analysis detected NSUN5 at an apparent molecular mass of 47 kDa.