NSUN4 Recombinant antibody

NSUN4 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241115G11

Cat no : 84275-3-RR

Synonyms

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115G11

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Validation Data Gallery

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  IF Staining of HepG2 using 84275-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 84275-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84275-3-RR is NSUN4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NSUN4 fusion protein Ag31828
• Full Name: NOL1/NOP2/Sun domain family, member 4
• Calculated Molecular Weight: 384 aa, 43 kDa
• GenBank Accession Number: BC014441
• Gene Symbol: NSUN4
• Gene ID (NCBI): 387338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.