NSUN4 Recombinant antibody, PBS Only

NSUN4 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241115B7

Cat no : 84275-1-PBS

Synonyms

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115B7

---

Validation Data Gallery

• 

  WB analysis using 84275-1-RR (same clone as 84275-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84275-1-RR (NSUN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84275-1-PBS in a different storage buffer formulation.

---

Product Information

The immunogen of 84275-1-PBS is NSUN4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NSUN4 fusion protein Ag31828
• Full Name: NOL1/NOP2/Sun domain family, member 4
• Calculated Molecular Weight: 384 aa, 43 kDa
• Observed Molecular Weight: ~43 kDa
• GenBank Accession Number: BC014441
• Gene Symbol: NSUN4
• Gene ID (NCBI): 387338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.