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NSUN2 Recombinant antibody, PBS Only

NSUN2 Recombinant Antibody for IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230186B7

Cat no : 82894-3-PBS

Synonyms

FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSUN2 Recombinant antibody (82894-3-RR)

    IF Staining of HepG2 using 82894-3-RR (same clone as 82894-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-3-RR, Clone: 230186B7 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82894-3-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82894-3-PBS targets NSUN2 in IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NSUN2 fusion protein Ag14791
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated Molecular Weight 767 aa, 86 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.