NSUN2 Recombinant antibody, PBS Only (Detector)

NSUN2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230186A6

Cat no : 82894-1-PBS

Synonyms

RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00112-3

  Sandwich ELISA standard curve of MP00112-3, Human NSUN2 Recombinant Matched Antibody Pair - PBS only. 82894-9-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14791. 82894-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/ml

• 

  Sample test of MP00112-3

  HCT-116 cell lysate was measured. The human NSUN2 concentration of detected samples was determined to be 15,374.1 pg/mL (based on a 3.0 mg/mL extract load).

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  IF Staining of HepG2 using 82894-1-RR (same clone as 82894-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-1-RR, Clone: 230186A6 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82894-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82894-1-RR (same clone as 82894-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human NSUN2 (82894-1-RR, Clone:230186A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82894-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00112-1

  Cytometric bead array standard curve of MP00112-1, NSUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82894-4-PBS. Detection antibody: 82894-1-PBS. Standard: Ag14791. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 82894-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82894-1-RR against Human NSUN2 were performed. The affinity constant is below 1 pM.

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Product Information

82894-1-PBS targets NSUN2 as part of a matched antibody pair:

MP00112-1: 82894-4-PBS capture and 82894-1-PBS detection (validated in Cytometric bead array)

MP00112-3: 82894-9-PBS capture and 82894-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NSUN2 fusion protein Ag14791
• Full Name: NOL1/NOP2/Sun domain family, member 2
• Calculated Molecular Weight: 767 aa, 86 kDa
• GenBank Accession Number: BC001041
• Gene Symbol: NSUN2
• Gene ID (NCBI): 54888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.