NSUN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NSUN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

76

Reviews (1)

Cat no : 20854-1-AP

Synonyms

SAKI, RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203

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Validation Data Gallery

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  WB analysis of SU-DHL-4 using 20854-1-AP

  WB result of NSUN2 antibody (20854-1-AP; 1:40000; room temperature for 1.5 hours) with wild-type and NSUN2 knockout SU-DHL-4 cells.

• 

  WB analysis using 20854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20854-1-AP

  WB result of NSUN2 antibody (20854-1-AP; 1:60000; room temperature for 1.5 hours) with sh-Control and sh-NSUN2 transfected HepG2 cells.

• 

  IP experiment of HeLa using 20854-1-AP

  IP result of anti-NSUN2 (IP:20854-1-AP, 4ug; Detection:20854-1-AP 1:1000) with HeLa cells lysate 4000ug.

• 

  IHC staining of human breast cancer using 20854-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20854-1-AP (NSUN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20854-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20854-1-AP (NSUN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 20854-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20854-1-AP (NSUN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 20854-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 20854-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 20854-1-AP

  Immunofluorescent analysis of HepG2 cells, using NSUN2 antibody 20854-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 20854-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NSUN2 (20854-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, HepG2 cells, SU-DHL-4 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 29 publications below
• WB: See 70 publications below
• IHC: See 17 publications below
• IF: See 13 publications below
• IP: See 6 publications below
• CoIP: See 1 publications below

Product Information

20854-1-AP targets NSUN2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSUN2 fusion protein Ag14791
• Full Name: NOL1/NOP2/Sun domain family, member 2
• Calculated Molecular Weight: 767 aa, 86 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC001041
• Gene Symbol: NSUN2
• Gene ID (NCBI): 54888
• RRID: AB_10693629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis; Authors - Sunyang Ying
human	WB	Nat Biotechnol; Identification of direct targets and modified bases of RNA cytosine methyltransferases.; Authors - Khoddami Vahid V
mouse	WB	Cell; The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.; Authors - Deniz Simsek
human	WB,IHC	Nat Cell Biol; 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.; Authors - Xin Chen
human	WB	Nat Protoc; Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.; Authors - Vahid Khoddami
human	WB	Mol Cell; Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach; Authors - Liang Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah (Verified Customer) (01-05-2021)

Works perfectly for WB. Tested on HeLa cells and HEK293T.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa and HEL293T cells

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