NSFL1C Polyclonal antibody

• Featured Product
• KD/KO Validated

NSFL1C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 15620-1-AP

Synonyms

NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C

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Validation Data Gallery

• 

  WB analysis of A431 using 15620-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15620-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15620-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15620-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15620-1-AP

  IP result of anti-NSFL1C (IP:15620-1-AP, 4ug; Detection:15620-1-AP 1:1000) with HEK-293 cells lysate 2640ug.

• 

  IHC staining of human lung cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, mouse liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

15620-1-AP targets NSFL1C in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSFL1C fusion protein Ag8040
• Full Name: NSFL1 (p97) cofactor (p47)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 47 kDa, 41 kDa
• GenBank Accession Number: BC002801
• Gene Symbol: NSFL1C
• Gene ID (NCBI): 55968
• RRID: AB_2878158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Altered cofactor regulation with disease-associated p97/VCP mutations.; Authors - Xiaoyi Zhang
human	WB	Cell Chem Biol; Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.; Authors - Xiaojun Wu
human	WB	J Biol Chem; Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.; Authors - Tao Wang
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KD Validated
mouse	WB	Evid Based Complement Alternat Med; Shenxian-Shengmai Oral Liquid Improves Sinoatrial Node Dysfunction through the PKC/NOX-2 Signaling Pathway.; Authors - Heng Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Julia (Verified Customer) (07-02-2019)

Clean and specific bands on Western blot at correct MW.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human