NSD1 Polyclonal antibody

NSD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27346-1-AP

Synonyms

ARA267, H3 K36 HMTase, H4 K20 HMTase, KMT3B, Lysine N methyltransferase 3B, NSD1, SOTOS, STO

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Validation Data Gallery

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  WB analysis using 27346-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27346-1-AP (NSD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27346-1-AP targets NSD1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSD1 fusion protein Ag26425
• Full Name: nuclear receptor binding SET domain protein 1
• Observed Molecular Weight: 267-310 kDa
• GenBank Accession Number: BC150628
• Gene Symbol: NSD1
• Gene ID (NCBI): 64324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSD1 (nuclear receptor SET (su(var)3-9, enhancer-of-zeste,trithorax) domain containing protein-1), which is isolated and characterized in 2001, belongs to the NSD protein lysine methyltransferase (KMT) family. This family has three members, NSD1 (KMT3B), NSD2 (WHSC1/MMSET) and NSD3 (WHSC1L1), which could regulate the expression of target genes through methylation of lysine 36 on histone H3(H3K36). NSD1 could bind various promoter elements to regulate transcription via interactions with H3K36 methylation and RNA polymerase II.