NRXN1 Polyclonal antibody

NRXN1 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 55051-1-AP

Synonyms

Hs.22998, KIAA0578, neurexin 1, Neurexin 1 alpha, Neurexin I alpha, NRXN1

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 55051-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55051-1-AP (NRXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 55051-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRXN1 antibody (55051-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 55051-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRXN1 antibody (55051-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55051-1-AP targets NRXN1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: neurexin 1
• Calculated Molecular Weight: 162 kDa
• Observed Molecular Weight: 160-170 kDa
• GenBank Accession Number: NM_004801
• Gene Symbol: NRXN1
• Gene ID (NCBI): 9378
• RRID: AB_10859257
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRXN1, also named as KIAA0578, belongs to the neurexin family. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. NRXN1 may mediate intracellular signaling. The antibody is specific to NRXN1 alpha.