NRSN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NRSN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17574-1-AP

Synonyms

C20orf98, neurensin 2, NRSN2

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Validation Data Gallery

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  WB analysis of human brain using 17574-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17574-1-AP (NRSN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17574-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17574-1-AP (NRSN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 17574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17574-1-AP (NRSN2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17574-1-AP (NRSN2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

17574-1-AP targets NRSN2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRSN2 fusion protein Ag10387
• Full Name: neurensin 2
• Calculated Molecular Weight: 204 aa, 22 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC001963
• Gene Symbol: NRSN2
• Gene ID (NCBI): 80023
• RRID: AB_2155379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Am J Cancer Res; NRSN2 promotes osteosarcoma cell proliferation and growth through PI3K/Akt/MTOR and Wnt/β-catenin signaling.; Authors - Ajimu Keremu ; KD Validated
human	WB	Biomed Pharmacother; Highly expressed NRSN2 is related to malignant phenotype in ovarian cancer.; Authors - Wenbin Tang ; KD Validated
human	WB, IHC	Dig Dis Sci; Down-Regulated NRSN2 Promotes Cell Proliferation and Survival Through PI3K/Akt/mTOR Pathway in Hepatocellular Carcinoma.; Authors - Xin Wang
human	WB	Int J Clin Exp Pathol; NRSN2 promotes non-small cell lung cancer cell growth through PI3K/Akt/mTOR pathway.; Authors - Xin-Yi Zhang