NRIP3 Polyclonal antibody

NRIP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15664-1-AP

Synonyms

C11orf14, NRIP3, NY SAR 105, Sarcoma antigen NY SAR 105

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Validation Data Gallery

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  WB analysis of MCF-7 using 15664-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15664-1-AP (NRIP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15664-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15664-1-AP (NRIP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse ovary using 15664-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15664-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15664-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 15664-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: mouse ovary tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15664-1-AP targets NRIP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRIP3 fusion protein Ag8097
• Full Name: nuclear receptor interacting protein 3
• Calculated Molecular Weight: 241 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC008835
• Gene Symbol: NRIP3
• Gene ID (NCBI): 56675
• RRID: AB_2878165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.