Neurogranin Polyclonal antibody

Neurogranin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10440-1-AP

Synonyms

hng, Neurogranin, Ng, NRGN, P17, RC3

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 10440-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10440-1-AP (Neurogranin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10440-1-AP

  IP result of anti-Neurogranin (IP:10440-1-AP, 4ug; Detection:10440-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of mouse brain using 10440-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10440-1-AP (Neurogranin antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10440-1-AP targets Neurogranin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neurogranin fusion protein Ag0676
• Full Name: neurogranin (protein kinase C substrate, RC3)
• Calculated Molecular Weight: 7.6 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC002835
• Gene Symbol: Neurogranin
• Gene ID (NCBI): 4900
• RRID: AB_2251586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.The observed molecular weight of human NRGN by Western blotting is about 15-17 kDa.

Publications

Species	Application	Title
mouse	IF	Cells; Specificity and Redundancy of Profilin 1 and 2 Function in Brain Development and Neuronal Structure.; Authors - Marina Di Domenico
mouse	IHC	Sci Rep; Hypoxia-inducible factor-2α is crucial for proper brain development.; Authors - Kira Kleszka
mouse		Elife; An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning.; Authors - Ingrid Ehrmann