Filter: AllIHCIF-PIF/ICCAffinity

IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 83323-6-RR (same clone as 83323-6-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 83323-6-RR (same clone as 83323-6-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.
IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.
IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 83323-6-RR (same clone as 83323-6-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83323-6-RR
Biolayer interferometry (BLl) kinetic assays of 83323-6-RR against Human NRG1, isoform SMDF were performed. The affinity constant is 1.64 nM.

NRG1, isoform SMDF Recombinant antibody, PBS Only

NRG1, isoform SMDF Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA

Cat No. 83323-6-PBS

Clone No.240184G2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IF-P, ELISA

Synonyms

NRG1, NRG1A, Heregulin, Glial growth factor, GGF2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

83323-6-PBS targets NRG1, isoform SMDF in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	NRG1, isoform SMDF fusion protein Ag0803 Predict reactive species
Full Name	neuregulin 1
Calculated Molecular Weight	70 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC007675
Gene Symbol	NRG1
Gene ID (NCBI)	3084
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q02297
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform SMDF.