Various lysates were subjected to SDS PAGE followed by western blot with 31043-1-AP (NRG1, isoform Alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 31043-1-AP (NRG1, isoform Alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IF Staining of MCF-7 using 31043-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform Alpha antibody (31043-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform Alpha antibody (31043-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Alpha.
Protocols
Product Specific Protocols
WB protocol for NRG1, isoform Alpha antibody 31043-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31043-1-AP (NRG1, isoform Alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IF/ICC Figures
IF Staining of MCF-7 using 31043-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform Alpha antibody (31043-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
NRG1, isoform Alpha Polyclonal antibody
Catalog Number
31043-1-AP
Citations
-
Dilutions
WB : 1:500-1:1000 IF/ICC : 1:200-1:800
Applications
WB, IF/ICC, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
