• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NRF2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, ChIP-Seq, WB

Activ Motif logo

Cat No : 61599,61600 61599

Synonyms

NFE2L2, Nuclear Factor Erythroid 2-Like 2, HEBP1, NF-E2-Related Factor 2, NFE2-Related Factor 2, NRF2, Nuclear Factor Erythroid 2-Related Factor 2, Nuclear Factor (Erythroid-Derived 2)-Like 2, Nuclear Factor Erythroid-Derived 2-Like 2, Nuclear Factor, Erythroid Derived 2, Like 2, transcription activator, are, antioxidant response element, antibody, antibodies, pab, polyclonal, chip, chromatin immunoprecipitation, chip-seq, western blotting, wb, sample

Validation Data Gallery

  • NRF2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from A549 cells and 4 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify NRF2 binding sites. The image shows binding across regions of chromosome 16 and 22. You can view the complete data set in the UCSC Genome Browser, starting at NQO1, here.

    NRF2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from A549 cells and 4 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify NRF2 binding sites. The image shows binding across regions of chromosome 16 and 22. You can view the complete data set in the UCSC Genome Browser, starting at NQO1, here.

  • NRF2 antibody (pAb) tested by Western blot. Nuclear extract of HepG2 cells (25 ug) treated with tHBQ and probed with NRF2 antibody at a dilution of 1:500.

    NRF2 antibody (pAb) tested by Western blot. Nuclear extract of HepG2 cells (25 ug) treated with tHBQ and probed with NRF2 antibody at a dilution of 1:500.

Product Information

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP: 10 ul per ChIP ChIP-Seq: 10 ul each WB: 1:500 - 1:1,000 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within human NRF2.
Full Name NRF2 antibody (pAb)
Synonyms NFE2L2, Nuclear Factor Erythroid 2-Like 2, HEBP1, NF-E2-Related Factor 2, NFE2-Related Factor 2, NRF2, Nuclear Factor Erythroid 2-Related Factor 2, Nuclear Factor (Erythroid-Derived 2)-Like 2, Nuclear Factor Erythroid-Derived 2-Like 2, Nuclear Factor, Erythroid Derived 2, Like 2, transcription activator, are, antioxidant response element, antibody, antibodies, pab, polyclonal, chip, chromatin immunoprecipitation, chip-seq, western blotting, wb, sample
Molecular weight 96 kDa
GenBank accession numberNP_006155
RRIDAB_2793695
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

NRF2 (NF-E2-Related Factor 2) is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.