CoraLite® Plus 488-conjugated NRF2, NFE2L2 Recombinant antibody

• Featured Product
• KD/KO Validated

NRF2, NFE2L2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1I21

Cat no : CL488-80593

Synonyms

NRF2, NFE2L2, HEBP1, Nrf 2, NFE2-related factor 2

---

Validation Data Gallery

• 

  IF Staining of HepG2 using CL488-80593

  Immunofluorescent analysis of (4% PFA) fixed MG132 treated HepG2 cells using CoraLite® Plus 488 NRF2, NFE2L2 antibody (CL488-80593, Clone: 1I21 ) at dilution of 1:200.

• 

  FC experiment of MCF-7 using CL488-80593

  1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NRF2, NFE2L2 (CL488-80593, Clone:1I21) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: MG132 treated HepG2 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80593 targets NRF2, NFE2L2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NRF2, NFE2L2 fusion protein Ag9489
• Full Name: nuclear factor (erythroid-derived 2)-like 2
• Calculated Molecular Weight: 605 aa, 68 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC011558
• Gene Symbol: NRF2
• Gene ID (NCBI): 4780
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011