NRD1 Polyclonal antibody

NRD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15630-1-AP

Synonyms

hNRD1, hNRD2, N arginine dibasic convertase, Nardilysin, NRD C, NRD convertase, NRD1

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Validation Data Gallery

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  WB analysis using 15630-1-AP

  K-562 cells lysates were subjected to SDS PAGE followed by western blot with 15630-1-AP (NRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 15630-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15630-1-AP (NRD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 15630-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15630-1-AP (NRD1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: mouse testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15630-1-AP targets NRD1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRD1 fusion protein Ag7923
• Full Name: nardilysin (N-arginine dibasic convertase)
• Calculated Molecular Weight: 132 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: BC008775
• Gene Symbol: NRD1
• Gene ID (NCBI): 4898
• RRID: AB_2154525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRD1(Nardilysin) is also named as NRD-C(N-arginine dibasic convertase). It is a zinc metalloendopeptidase of the M16 pitrilysin family that cleaves peptides at dibasic residues. The enzyme has also been found in neural tissues of mouse embryos indicating a role in neural development. Although nardilysin is primarily found in the cytosol, it has been reported that nardilysin is located on the cell surface acting as a receptor for heparinbinding EGF-like growth factor(PMID:15809022). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	IF	Diabetes; Nardilysin-dependent proteolysis of cell-associated VTCN1 (B7-H4) marks type 1 diabetes development.; Authors - Ilian A Radichev
mouse	WB	J Immunol; Loss of Peripheral Protection in Pancreatic Islets by Proteolysis-Driven Impairment of VTCN1 (B7-H4) Presentation Is Associated with the Development of Autoimmune Diabetes.; Authors - Ilian A Radichev
human	IHC	J Clin Med; Serum Nardilysin as a Prognostic Biomarker in Pancreatic Ductal Adenocarcinoma.; Authors - Yongfeng Xu