NRCAM Polyclonal antibody

• Featured Product
• KD/KO Validated

NRCAM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 21608-1-AP

Synonyms

Ng-CAM-related, Ng CAM related, Neuronal surface protein Bravo, neuronal cell adhesion molecule, KIAA0343

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Validation Data Gallery

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  WB analysis using 21608-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21608-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21608-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP (NRCAM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP (NRCAM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21608-1-AP (NRCAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21608-1-AP (NRCAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 21608-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 21608-1-AP (NRCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, HEK-293 cells, rat brain tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

21608-1-AP targets NRCAM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRCAM fusion protein Ag16237
• Full Name: neuronal cell adhesion molecule
• Calculated Molecular Weight: 1304 aa, 144 kDa
• Observed Molecular Weight: 130-136 kDa
• GenBank Accession Number: BC098401
• Gene Symbol: NRCAM
• Gene ID (NCBI): 4897
• RRID: AB_10859786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).

Publications

Species	Application	Title
human	IF	Stem Cell Reports; CD73 Regulates Stemness and Epithelial-Mesenchymal Transition in Ovarian Cancer-Initiating Cells.; Authors - Michela Lupia
human	WB	Mol Cell Proteomics; Application of mass spectrometry profiling to establish brusatol as an inhibitor of global protein synthesis.; Authors - Steffan Vartanian
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin
human	WB	Cancer Gene Ther; NrCAM secreted by endometrial stromal cells enhances the progestin sensitivity of endometrial cancer cells through epigenetic modulation of PRB.; Authors - Yali Cheng ; KD Validated
mouse	IHC	Mol Cell Neurosci; Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.; Authors - Zhen-Huang Chen