NR5A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NR5A2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 22460-1-AP

Synonyms

B1 binding factor, B1F, B1F2, CPF, CYP7A promoter binding factor, FTF, FTZ F1, FTZ F1beta, hB1F, hB1F 2, Liver receptor homolog 1, LRH 1, NR5A2

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Validation Data Gallery

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  WB analysis using 22460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22460-1-AP (NR5A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 22460-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22460-1-AP (NR5A2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 22460-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 22460-1-AP (NR5A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, rat testis tissue, mouse ovary tissue, BxPC-3 cells, mouse liver tissue, mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

22460-1-AP targets NR5A2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR5A2 fusion protein Ag18001
• Full Name: nuclear receptor subfamily 5, group A, member 2
• Calculated Molecular Weight: 541 aa, 61 kDa
• Observed Molecular Weight: 61-67 kDa
• GenBank Accession Number: BC118652
• Gene Symbol: NR5A2
• Gene ID (NCBI): 2494
• RRID: AB_2879108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR5A2 belongs to the nuclear hormone receptor family NR5 subfamily.It binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation.NR5A2 may be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. It may also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. LRH-1 is phosphorylated to display its normal function, including its role in the intriguing potential responses to phospholipid or other ligands. The hinge domain serine residues at 238 and 243 are required for effective phosphorylation, both in vitro and in cells (16439367).NR5A1 is abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in hepG2. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and hepG2 cells.The observed molecular weight of 65-70 kDa is the posphorylation of NR5A2 protein.

Publications

Species	Application	Title
mouse	WB	Aging (Albany NY); NR5A2 as a potential target for exercise to improve metabolic syndrome; Authors - Lingxiu Meng
human,mouse	WB,IHC,IF	Cell Prolif; LRH-1 activation alleviates diabetes-induced podocyte injury by promoting GLS2-mediated glutaminolysis; Authors - Jijia Hu
human	WB	Mol Ther Nucleic Acids; MicroRNA-3613-5p Promotes Lung Adenocarcinoma Cell Proliferation through a RELA and AKT/MAPK Positive Feedback Loop.; Authors - Tao He ; KD Validated
mouse	WB	Pharmacol Res; Geniposide reduces cholesterol accumulation and increases its excretion by regulating the FXR-mediated liver-gut crosstalk of bile acids.; Authors - Jinxin Liu
human	WB,IHC	Front Oncol; NR5A2 Is One of 12 Transcription Factors Predicting Prognosis in HNSCC and Regulates Cancer Cell Proliferation in a p53-Dependent Manner.; Authors - Kun Zhang ; KD Validated
human	WB, IHC	Cell Death Discov; NR5A2 transcriptional activation by BRD4 promotes pancreatic cancer progression by upregulating GDF15.; Authors - Feng Guo