NR4A3 Polyclonal antibody, PBS Only

• 

  WB analysis of mouse spleen using 55405-1-AP (same clone as 55405-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55405-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55405-1-AP (same clone as 55405-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55405-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 55405-1-AP (same clone as 55405-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55405-1-PBS in a different storage buffer formulation.

• 

  IF Staining of ROS1728 using 55405-1-AP (same clone as 55405-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed ROS1728 cells using NR4A3 antibody (55405-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55405-1-PBS in a different storage buffer formulation.

NR4A3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 55405-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TEC, NOR1, MINOR, CSMF, CHN

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Product Information

55405-1-PBS targets NR4A3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear receptor subfamily 4, group A, member 3
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_006981
• Gene Symbol: NR4A3
• Gene ID (NCBI): 8013
• RRID: AB_2881322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92570
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nuclear receptor 4A3 (NR4A3, also known as NOR-1), NR4A1 and NR4A2 are TFs belonging to NR4A orphan nuclear receptor subfamily. The role of all three NR4A members in regulating gene expression involved in cell proliferation, differentiation, apoptosis, inflammation and metabolism in cardiovascular diseases has been widely studied. In osteoblasts, NR4A receptor has been proved to regulate the expression and differentiation of osteoblast genes. It is reported that NR4A receptor is highly expressed in human coronary atherosclerotic lesions, and NR4A3 deficiency can reduce the proliferation and migration of VSMCs and the formation of neointima after vascular injury. Therefore, NR4A3 may play an important role in mediating phenotypic transformation of VSMC (PMID: 38629274). It is expected to be located in nucleus. The protein has three isforms, isoform alpha is highly expressed in skeletal muscle. Isoform beta is highly expressed in skeletal muscle and low expressed in fetal brain and placenta. The calculated molecular weight of NR4A3 is 68 kDa.