NR4A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NR4A1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 25851-1-AP

Synonyms

Early response protein NAK1, GFRP1, HMR, N10, NAK 1, NAK1, NGFIB, NR4A1, NUR77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST 59, Testicular receptor 3, TR3

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Validation Data Gallery

• 

  WB analysis of PC-3 using 25851-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25851-1-AP (NR4A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25851-1-AP (NR4A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25851-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25851-1-AP (NR4A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25851-1-AP

  IP result of anti-NR4A1 (IP:25851-1-AP, 4ug; Detection:25851-1-AP 1:500) with HeLa cells lysate 1720 ug.

• 

  IHC staining of mouse colon using 25851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25851-1-AP (NR4A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 25851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25851-1-AP (NR4A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, PC-3 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

25851-1-AP targets NR4A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR4A1 fusion protein Ag23088
• Full Name: nuclear receptor subfamily 4, group A, member 1
• Calculated Molecular Weight: 598 aa, 64 kDa
• Observed Molecular Weight: 64-68 kDa
• GenBank Accession Number: BC016147
• Gene Symbol: NR4A1
• Gene ID (NCBI): 3164
• RRID: AB_2880269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Cancer Lett; NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathway; Authors - Jinghan Zhu
mouse	WB	FASEB J; The role of nuclear receptor 4A1 (NR4A1) in drug-induced gingival overgrowth.; Authors - Saki Hatano
mouse	WB,IHC	J Cell Mol Med; NR4A1 retards adipocyte differentiation or maturation via enhancing GATA2 and p53 expression.; Authors - Dan-Dan Qin ; KO Validated
rat	IF	Phytomedicine; Stigmasterol upregulates immediate early genes and promotes neuronal cytoarchitecture in primary hippocampal neurons as revealed by transcriptome analysis.; Authors - Md Nazmul Haque
human	WB	Front Mol Biosci; Single-Cell Profiles of Age-Related Osteoarthritis Uncover Underlying Heterogeneity Associated With Disease Progression.; Authors - Wenzhou Liu ; KD Validated
mouse	WB	Am J Physiol Endocrinol Metab; Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring.; Authors - Xiaoyan Wan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angela (Verified Customer) (06-23-2020)

35ug total protein/lane1- Untransfected HEK2932- Transfection with human NR4A1 expression vector3- Transfection with rat NR4A1 expression vector

• Applications: Western Blot
• Primary Antibody Dilution: 500x
• Cell Tissue Type: HEK293

NR4A1 Antibody Western Blot validation (500x dilution) in HEK293 (Cat no:25851-1-AP)