PXR Polyclonal antibody

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  WB analysis of MCF-7 using 15607-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15607-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15607-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 15607-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

PXR Polyclonal Antibody for ELISA

Cat No. 15607-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Synonyms

BXR, NR1I2, ONR1, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, PAR, PAR1, PAR2, PARq, Pregnane X receptor, PRR, PXR, SAR, SXR

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Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

15607-1-AP targets PXR in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PXR fusion protein Ag7991
• Full Name: nuclear receptor subfamily 1, group I, member 2
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC017304
• Gene Symbol: PXR
• Gene ID (NCBI): 8856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75469
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, plays a key role in the induction of genes involved in drug transport and metabolism. It modulates drug transport and metabolism through the regulation of target genes responsible for the transport and conversion of chemicals into metabolites that are more easily eliminated from the body [PMID:22609277]. It is also emerging as an endobiotic receptor that regulates the inflammatory response. In addition, PXR acts as a transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds [PMID: 19297428].

Publications

Species	Application	Title
human	IF,WB	Hepatol Int; Hepatitis B virus-related intrahepatic cholangiocarcinoma originates from hepatocytes; Authors - Zimin Song
mouse	CoIP	Cell Death Dis; Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.; Authors - Cheng Wang ; KD Validated
human	WB	Biochem Pharmacol; Constitutive androstane receptor weakens the induction of panaxytriol on CYP3A4 by repressing the activation of pregnane X receptor.; Authors - Qingqing Hu
mouse	WB	Br J Nutr; Retinoic acid induces macrophage cholesterol efflux and inhibits atherosclerotic plaque formation in apoE-deficient mice.; Authors - Wenjing Zhou
human	WB,IHC	Ann Transl Med; Curcuma zedoaria petroleum ether extract reverses the resistance of triple-negative breast cancer to docetaxel via pregnane X receptor.; Authors - En-Qi Qiao
human	IHC	Reprod Sci; Protease Amplification of the Inflammatory Response Induced by Commensal Bacteria: Implications for Racial Disparity in Term and Preterm Birth.; Authors - Scott W Walsh