NR1D2 Polyclonal antibody

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  WB analysis of Jurkat using 13906-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13906-1-AP (NR1D2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13906-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13906-1-AP (NR1D2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13906-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13906-1-AP (NR1D2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 13906-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13906-1-AP (NR1D2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 13906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using NR1D2 antibody (13906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

NR1D2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13906-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Reverb Beta, Rev-erb alpha-related receptor, Rev erb beta, EAR-1R, EAR 1R

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

13906-1-AP targets NR1D2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR1D2 fusion protein Ag4881
• Full Name: nuclear receptor subfamily 1, group D, member 2
• Calculated Molecular Weight: 579 aa, 65 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC045613
• Gene Symbol: NR1D2
• Gene ID (NCBI): 9975
• RRID: AB_2877986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14995
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Sci Rep; The functional and predictive roles of miR-210 in cryptorchidism.; Authors - Zhengzheng Duan
mouse	WB	Am J Physiol Heart Circ Physiol; Differential effects of REV-ERBα/β agonism on cardiac gene expression, metabolism, and contractile function in a mouse model of circadian disruption.; Authors - Sobuj Mia
human	WB	Xenobiotica; Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells.; Authors - Min Chen
human	WB	Cell Death Dis; SR9009 inhibits lethal prostate cancer subtype 1 by regulating the LXRα/FOXM1 pathway independently of REV-ERBs; Authors - Hang Xu ; KD Validated
human,rat	WB	NPJ Biofilms Microbiomes; Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling; Authors - Shang Li
mouse	IHC	J Biomed Sci; Secretome from estrogen-responding human placenta-derived mesenchymal stem cells rescues ovarian function and circadian rhythm in mice with cyclophosphamide-induced primary ovarian insufficiency; Authors - Duy-Cuong Le