NR1D1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NR1D1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14506-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

  WB result of anti-NR1D1 (14506-1-AP,1:1000) with A549 cell by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

  WB result of anti-NR1D1 (14506-1-AP,1:500) with A549 treated with Cycloheximide by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A549 using 14506-1-AP (same clone as 14506-1-PBS)

  IP result of anti-NR1D1 (IP:14506-1-AP, 4ug; Detection:14506-1-AP 1:300) with A549 cells lysate 1200ug. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 14506-1-AP (same clone as 14506-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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Product Information

14506-1-PBS targets NR1D1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR1D1 fusion protein Ag5964
• Full Name: nuclear receptor subfamily 1, group D, member 1
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 55-68 kDa
• GenBank Accession Number: BC056148
• Gene Symbol: NR1D1
• Gene ID (NCBI): 9572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.