NR1D1 antibody (14506-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 14506-1-AP (same clone as 14506-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.
WB analysis using 14506-1-AP (same clone as 14506-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.
WB analysis using 14506-1-AP (same clone as 14506-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.
WB analysis using 14506-1-AP (same clone as 14506-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.
WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)
WB result of anti-NR1D1 (14506-1-AP,1:1000) with A549 cell by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.
WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)
WB result of anti-NR1D1 (14506-1-AP,1:500) with A549 treated with Cycloheximide by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 14506-1-AP (same clone as 14506-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

14506-1-PBS targets NR1D1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NR1D1 fusion protein Ag5964 Predict reactive species
Full Name	nuclear receptor subfamily 1, group D, member 1
Calculated Molecular Weight	67 kDa
Observed Molecular Weight	55-68 kDa
GenBank Accession Number	BC056148
Gene Symbol	NR1D1
Gene ID (NCBI)	9572
RRID	AB_11182604
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20393
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.