NQO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NQO1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

345

Cat no : 11451-1-AP

Synonyms

DTD, DT diaphorase, DIA4, DIA 4, DHQU

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Validation Data Gallery

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  WB analysis using 11451-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11451-1-AP (NQO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11451-1-AP

  WB result of NQO1 antibody (11451-1-AP, 1:1000) with si-control and si-NQO1 transfected HepG2 cells.

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  IP experiment of HepG2 using 11451-1-AP

  IP result of anti-NQO1 (IP:11451-1-AP, 4ug; Detection:11451-1-AP 1:300) with HepG2 cells lysate 3000 ug.

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  IHC staining of human colon cancer using 11451-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11451-1-AP (NQO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11451-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NQO1 antibody (11451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 11451-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human NQO1 (11451-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, LNCaP cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 327 publications below
• IHC: See 25 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Product Information

11451-1-AP targets NQO1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, rabbit, chicken, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NQO1 fusion protein Ag2009
• Full Name: NAD(P)H dehydrogenase, quinone 1
• Calculated Molecular Weight: 274 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC007659
• Gene Symbol: NQO1
• Gene ID (NCBI): 1728
• RRID: AB_2298729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.

Publications

Species	Application	Title
human	WB	Nature; The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.; Authors - Kirill Bersuker ; KO Validated
mouse	WB	Sci Transl Med; Activation of NRF2 ameliorates oxidative stress and cystogenesis in autosomal dominant polycystic kidney disease.; Authors - Yi Lu
human	WB	Mol Cell; Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.; Authors - Alessandra Fiore
mouse	WB	Nat Commun; Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.; Authors - Insup Choi
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
pig	WB	Microbiome; Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.; Authors - Leli Wang

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