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NQO1 Polyclonal antibody
NQO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11451-1-AP
|Positive WB detected in||HepG2 cells, K-562 cells, LNCaP cells, MCF-7 cells, NIH/3T3 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11451-1-AP targets NQO1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, goat, chicken, pig, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NQO1 fusion protein Ag2009|
|Full Name||NAD(P)H dehydrogenase, quinone 1|
|Calculated molecular weight||274 aa, 31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC007659|
|Gene ID (NCBI)||1728|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 ) This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.
|Product Specific Protocols|
|WB protocol for NQO1 antibody 11451-1-AP||Download protocol|
|IHC protocol for NQO1 antibody 11451-1-AP||Download protocol|
|IF protocol for NQO1 antibody 11451-1-AP||Download protocol|
|IP protocol for NQO1 antibody 11451-1-AP||Download protocol|
|FC protocol for NQO1 antibody 11451-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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