NPTX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NPTX1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 20656-1-AP

Synonyms

NP-I, NP1, Neuronal pentraxin-1, Neuronal pentraxin I

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Validation Data Gallery

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  WB analysis using 20656-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20656-1-AP (NPTX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20656-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20656-1-AP (NPTX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20656-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20656-1-AP (NPTX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 20656-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20656-1-AP (NPTX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 20656-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20656-1-AP (NPTX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20656-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20656-1-AP (NPTX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20656-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20656-1-AP (NPTX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 20656-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NPTX1 antibody (20656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 20656-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NPTX1 antibody (20656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20656-1-AP

  Immunofluorescent analysis of Hela cells, using NPTX1 antibody 20656-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells, mouse liver tissue, mouse cerebellum tissue, rat cerebellum tissue
• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

20656-1-AP targets NPTX1 in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: neuronal pentraxin I
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_002522
• Gene Symbol: NPTX1
• Gene ID (NCBI): 4884
• RRID: AB_10793669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPTX1, also named as NP1, may mediate uptake of degraded synaptic material which could play an important role in synaptic remodeling. NPTX1 can mediate the neuronal and glial uptake of the snake venom toxin taipoxin. The antibody is specific to NPTX1.

Publications

Species	Application	Title
mouse	WB, CoIP	Front Immunol; Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice.; Authors - Réka Á Kovács
	WB	Acta Biochim Biophys Sin (Shanghai); miR-139-5p mediates the palmitate-induced inhibition of insulin secretion by targeting neuronal pentraxin 1 in INS-1 cells.; Authors - Kai Zhang
mouse	IF	PLoS One; RNA-seq analysis of gene expression profiles in isolated stria vascularis from wild-type and Alport mice reveals key pathways underling Alport strial pathogenesis.; Authors - Brianna Dufek
human	WB	Cancer Manag Res; NPTX1 promotes metastasis via integrin/FAK signaling in gastric cancer.; Authors - Hongfei Yan ; KD Validated
human	WB	PLoS One; Exosome-delivered circRPS5 inhibits the progression of melanoma via regulating the miR-151a/NPTX1 axis; Authors - Haijun Zhu ; KD Validated
mouse	WB	Oncogene; Chromatin-associated OGT promotes the malignant progression of hepatocellular carcinoma by activating ZNF263; Authors - Lingyan Wang

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