NPTN Polyclonal antibody, PBS Only

NPTN Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28022-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of C6 using 28022-1-AP (same clone as 28022-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 28022-1-AP (NPTN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28022-1-PBS in a different storage buffer formulation.

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Product Information

28022-1-PBS targets NPTN in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPTN fusion protein Ag27752
• Full Name: neuroplastin
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC030773
• Gene Symbol: NPTN
• Gene ID (NCBI): 27020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Neuroplastin (Nptn) is a member of the Ig superfamily and is expressed in two isoforms, Np55 and Np65. Np65 contains three Ig domains (Ig1, Ig2, Ig3). Np55 contains only two (Ig2, Ig3). Np65 regulates synaptic transmission but the function of Np55 is unknown. Expression of the 65 kD neuroplastin (Np65) isoform derived by alternative splicing from the Nptn gene is restricted to brain neurons, whereas the 55 kD isoform (Np55) is present in most cell types but not in glia. (PMID: 33844052, PMID: 33299977, PMID: 27503893,PMID: 27581460)