NPR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NPR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55116-1-AP

Synonyms

ANP A, ANPa, ANPR A, ANPRA, GC A, Guanylate cyclase A, GUC2A, GUCY2A, NPR1

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Validation Data Gallery

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  WB analysis of SGC-7901 using 55116-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 55116-1-AP (NPR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 55116-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55116-1-AP (NPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55116-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55116-1-AP (NPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SGC-7901 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

55116-1-AP targets NPR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 119 kDa
• GenBank Accession Number: NM_000906
• Gene Symbol: NPR1
• Gene ID (NCBI): 4881
• RRID: AB_2881271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Biol Chem; 17β-Estradiol nongenomically induces vascular endothelial H2S release by promoting phosphorylation of cystathionine γ-lyase.; Authors - Xingyan Xu
human	WB	Int J Clin Exp Med; Impact of NPR-A expression in gastric cancer cells.; Authors - Jia Zhang ; KD Validated