NPPA Polyclonal antibody

NPPA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

42

Reviews (3)

Cat no : 27426-1-AP

Synonyms

ANF, ANP, ATFB6, Atrial natriuretic factor, Atrial natriuretic peptide, CDD ANF, CDP, NPPA, PND, Prepronatriodilatin

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Validation Data Gallery

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  WB analysis using 27426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 27426-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 27426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 27426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 27426-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 27426-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of C2C12 using 27426-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using NPPA antibody (27426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 27426-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NPPA antibody (27426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 41 publications below
• IHC: See 1 publications below
• ELISA: See 1 publications below

Product Information

27426-1-AP targets NPPA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPPA fusion protein Ag26692
• Full Name: natriuretic peptide precursor A
• Calculated Molecular Weight: 153 aa, 17 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC005893
• Gene Symbol: NPPA
• Gene ID (NCBI): 4878
• RRID: AB_2880868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Natriuretic peptide precursor-A (NPPA) is an early and specific marker for functional myocardium of the embryonic heart. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. NPPA is expressed primarily in the heart, where the expression level is higher in atria than ventricles. Low levels of ANP expression have been detected in other tissues, including the lung, aorta, brain, adrenal gland, kidney and uterus.

Publications

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p.; Authors - Xudong Zhang
rat	WB	Biochim Biophys Acta Mol Basis Dis; Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ; Authors - Yang Zhou
human,mouse	WB	Pharmacol Res; Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.; Authors - Xingda Li
rat	WB	J Cell Mol Med; The total xanthones extracted from Gentianella acuta alleviates HFpEF by activating the IRE1α/Xbp1s pathway; Authors - Linna Zhao
mouse	WB	ACS Omega; Gastrodin Exerts Cardioprotective Action via Inhibition of Insulin-Like Growth Factor Type 2/Insulin-Like Growth Factor Type 2 Receptor Expression in Cardiac Hypertrophy.; Authors - Jun Lu
mouse	WB	J Cell Physiol; PMID: 30488495; Authors - Jiali Nie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

Weak but smear band might need high resolution gels

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart

FH

Yan (Verified Customer) (08-19-2020)

It worked when I performed the immunostaining in neonatal mouse ventricular cardiomyocytes culture. Hypertrophy stimulus induced its expression.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: neonatal mouse ventricular cardiomyocytes culture

FH

Jie (Verified Customer) (03-06-2020)

IHC shoes a positive staining in the nuclei

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: cardiomyocyte culture

NPPA Antibody Immunohistochemistry, validation (1:200 dilution) in cardiomyocyte culture (Cat no:27426-1-AP)