NPHP1 Polyclonal antibody

NPHP1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25971-1-AP

Synonyms

JBTS4, Nephrocystin 1, nephronophthisis 1 (juvenile), NPHP1, SLSN1

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Validation Data Gallery

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  IHC staining of mouse testis using 25971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25971-1-AP (NPHP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 25971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25971-1-AP (NPHP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25971-1-AP targets NPHP1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPHP1 fusion protein Ag22965
• Full Name: nephronophthisis 1 (juvenile)
• Calculated Molecular Weight: 83 kDa
• GenBank Accession Number: BC062574
• Gene Symbol: NPHP1
• Gene ID (NCBI): 4867
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.