NPFFR2 Polyclonal antibody

NPFFR2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 19505-1-AP

Synonyms

G protein coupled receptor 74, GPR74, HLWAR77, neuropeptide FF receptor 2, NPFFR2

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Validation Data Gallery

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  IHC staining of human small intestine using 19505-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 19505-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 19505-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 19505-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 19505-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 19505-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human small intestine tissue, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19505-1-AP targets NPFFR2 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: neuropeptide FF receptor 2
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_053036
• Gene Symbol: NPFFR2
• Gene ID (NCBI): 10886
• RRID: AB_2878584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPFFR2, also named as GPR74, NPFF2 and NPGPR, belongs to the G-protein coupled receptor 1 family. NPFFR2 is a receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides which also known as morphine-modulating peptides. NPFFr2 can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. NPFFR2 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The antibody can recognize isoform1, isoform2 and isoform3 of NPFFR2.