NPEPPS Polyclonal antibody

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  WB analysis using 31633-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31633-1-AP (NPEPPS antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 31633-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NPEPPS antibody (31633-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

NPEPPS Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31633-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

AAP S, Cytosol alanyl aminopeptidase, MP100, NPEPPS, PSA

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Tested Applications

• Positive WB detected in: A431 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31633-1-AP targets NPEPPS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36035

  Product name: Recombinant human NPEPPS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 711-919 aa of BC065294

  Sequence: GKLGKAGHKATLEEARRRFKDHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLGATLLPDLIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAESIHQYLLQRKASPPTV

• Full Name: aminopeptidase puromycin sensitive
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: BC065294
• Gene Symbol: NPEPPS
• Gene ID (NCBI): 9520
• RRID: AB_3670057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P55786
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPEPPS, also known as PSA, is a member of the oxytocinase subfamily of the M1 aminopeptidase family. NPEPPS encodes puromycin-sensitive aminopeptidase, a zinc metallopeptidase that hydrolyzes amino acids from the N-terminus of its substrate. The protein has 2 isoforms and is located in the cytoplasm.