Nucleoside phosphorylase Polyclonal antibody

Nucleoside phosphorylase Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18009-1-AP

Synonyms

Inosine phosphorylase, NP, nucleoside phosphorylase, PNP, PRO1837, PUNP

---

Validation Data Gallery

• 

  WB analysis using 18009-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18009-1-AP (Nucleoside phosphorylase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18009-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18009-1-AP (Nucleoside phosphorylase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 18009-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 18009-1-AP (Nucleoside phosphorylase antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Jurkat cells, U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

18009-1-AP targets Nucleoside phosphorylase in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nucleoside phosphorylase fusion protein Ag12507
• Full Name: nucleoside phosphorylase
• Calculated Molecular Weight: 289 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC106074
• Gene Symbol: Purine nucleoside phosphorylase
• Gene ID (NCBI): 4860
• RRID: AB_2034971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Hazard Mater; Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.; Authors - Ye Zhu
canine	WB	Biologicals; CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells; Authors - Zhenbin Liu
rat	IF,WB	Int Immunopharmacol; Spermidine attenuates monocrotaline-induced pulmonary arterial hypertension in rats by inhibiting purine metabolism and polyamine synthesis-associated vascular remodeling; Authors - Yu-Jing Chen
human,mouse	WB	Cell; De novo and salvage purine synthesis pathways across tissues and tumors; Authors - Diem H Tran
human,mouse	WB	J Ethnopharmacol; Diosmetin-7-O-β-D-glucopyranoside from Pogostemonis Herba alleviated SARS-CoV-2-induced pneumonia by reshaping macrophage polarization and limiting viral replication; Authors - Yun-Lu Xu